Plant Chromosome Engineering
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Plant Chromosome Engineering

Methods and Protocols
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James A. Birchler
776 g
270x184x20 mm
701, Methods in Molecular Biology

Focuses on vital techniques which can lead to the production of improved food, feed, fuel, fiber, and pharmaceuticals
Contains protocols tested and proven by expert researchers in the field
Features the product of the contributors experience in the form of key tips for the successful implementation of the methods
1. Recent Advances in Plant Transformation
Shyamkumar Barampuram and Zhanyuan J. Zhang

2. Engineering the Plastid Genome of Nicotiana sylvestris, a Diploid Model Species for Plastid Genetics
Pal Maliga and Zora Svab

3. Homologous Recombination in Plants: An Anti-Review
Michal Lieberman-Lazarovich and Avraham A. Levy

4. Chromosome Painting for Plant Biotechnology Akio Kato, Jonathan C. Lamb, Patrice S. Albert, Tatiana Danilova, Fangpu Han, Zhi Gao, Seth Findley, and James A. Birchler

5. Plant B Chromosomes
Andreas Houben, Shuhei Nasuda, and Takashi R. Endo

6. Telomere Truncation in Plants
Chunhui Xu and Weichang Yu

7. Engineered Plant Minichromosomes
Robert T. Gaeta and Lakshminarasimhan Krishnaswamy

8. Method for Bxb1-Mediated Site-Specific Integration In planta
Yuan-Yeu Yau, Yueju Wang, James G. Thomson, and David W. Ow

9. Targeted Mutagenesis in Arabidopsis Using Zinc Finger Nucleases
Feng Zhang and Daniel F. Voytas

10. Vectors and Methods for Hairpin RNA and Artificial microRNA-Mediated Gene Silencing in Plants
Andrew L. Eamens and Peter M. Waterhouse

11. Recombinant Protein Expression in Nicotiana
Nobuyuki Matoba, Keith R. Davis, and Kenneth E. Palmer

12. Chromosome Analysis and Sorting Using Flow Cytometry
Jaroslav Dolezel, Marie Kubaláková, Jarmila Cíhalíková, Pavla Suchánková, and Hana Simková

13. Super-Stretched Pachytene Chromosomes for Plant Molecular Cytogenetic Mapping
Dal-Hoe Koo and Jiming Jiang

14. Cytological Dissection of the Triticeae Chromosomes by the Gametocidal System
Takashi R. Endo

15. Development and Use of Oat-Maize Chromosome Additions and Radiation Hybrids
Ralf G. Kynast and Oscar Riera-Lizarazu

16. Enhancer Trapping in Plants
Sivanandan Chudalayandi

17. Chromatin Beacons: Global Sampling of Chromatin Physical Properties Using Chromatin Charting Lines
Aniça Amini, Chongyuan Luo, and Eric Lam

18. Transposable Elements as Catalysts for Chromosome Rearrangements
Jianbo Zhang, Chuanhe Yu, Lakshminarasimhan Krishnaswamy, and Thomas Peterson
With an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biology(TM) series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.