Toll-Like Receptors
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Toll-Like Receptors

Methods and Protocols
 Previously published in hardcover
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Previously published in hardcover
Claire E. McCoy
871 g
254x178x25 mm

Toll-Like Receptors are invaluable in the fight against infection and can lead to the pathogenesis of disease. This reference details procedures on how to identify, characterize and measure Toll-like receptor expression and downstream signaling.
An easily accessible reference volume for procedures on how to identify, characterize and measure Toll-like receptor expression and downstream signalling
Part I: Methods to Detect and Analyze Toll-Like Receptors

1 Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells
Jakub Siednienko and Sinead M. Miggin

2 Ligands, Cell-Based Models and Readouts Required for Toll-Like Receptor Action
Jérôme Dellacasagrande

3 Toll-Like Receptor Interactions Imaged by FRET Microscopy and GFP Fragment Reconstitution
Gabor Horvath, Scott Young, and Eicke Latz

4 Predicting Toll-Like Receptor Structures and Characterizing Ligand Binding
Joshua N. Leonard, Jessica K. Bell, and David M. Segal

5 Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures
Tom P. Monie, Nicholas J. Gay, and Monique Gangloff

6 Expression, Purification and Crystallization of Toll/Interleukin-1 Receptor (TIR) Domains
Xiao Tao and Liang Tong
Part II: Methods to Analyze Signal Transduction Downstream of Toll-Like Receptor Stimulation

7 Proteomic Analysis of Protein Complexes in Toll-Like Receptor Biology
Kiva Brennan and Caroline A. Jefferies

8 2D-DIGE Comparative Proteomics: Investigation of the Proteome in Cellular Signalling Pathways
Nadia Ben Larbi and Caroline A. Jefferies

9 MAPPIT (Mammalian Protein-Protein Interaction Trap) Analysis of Early Steps in Toll-Like Receptor Signalling
Peter Ulrichts, Irma Lemmens, Delphine Lavens, Rudi Beyaert, and Jan Tavernier

10 Analysis of the Functional Role of Toll-Like Receptor-4 Tyrosine Phosphorylation
Andrei E. Medvedev and Wenji Piao

11 Analysis of Ubiquitin Degradation and Phosphorylation of Proteins
Pearl Gray

12 The Generation of Highly Purified Primary Human Neutrophils and Assessment of Apoptosis in Response to Toll-Like ReceptorLigands
Lisa C. Parker, Lynne R. Prince, David J. Buttle, and Ian Sabroe

13 Cellular Expression of A20 and ABIN-3 in Response to Toll-Like Receptor-4 Stimulation
Kelly Vershelst, Lynn Verstrepen, Beatrice Coornaert, Isabelle Carpentier, and Rudi Beyaert

14 Characterisation of Viral Proteins that Inhibit Toll-Like Receptor Signal Transduction
Julianne Stack and Andrew G. Bowie
Part III: Genetic Techniques in Toll-Like Receptor Analysis

15 Genetic Dissection of Toll-Like Receptor Signaling Using ENU Mutagenesis
Kasper Hoebe

16 Microarray Experiments to Uncover Toll-Like Receptor Function
Harry Björkbacka

17 Uncovering Novel Gene Function in Toll-Like Receptor Signalling Using siRNA
Michael Carty, Sinéad Keating, and Andrew G. Bowie

18 Genotyping Methods to Analyze Polymorphisms in Toll-Like Receptors and Disease
Chiea-Chuen Khor
Part IV: Toll-Like Receptors and Disease

19 Experimental Models of Acute Infection and Toll-Like Receptor Driven Septic Shock
Ruth Ferstl, Stephan Spiller, Sylvia Fichte, Stefan Dreher, and Carsten J. Kirschning

20 Toll-Like Receptors and Rheumatoid Arthritis
Fabia Brentano, Diego Kyburz, and Steffen Gay

21 Practical Techniques for Detection of Toll-Like Receptor-4 in the Human Intestine
Ryan Ungaro, Maria T. Abreu, and Masayuki Fukata

22 Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells
Melissa B. Uccellini, Ana M. Avalos, Ann Marshak-Rothstein, and Gregory A. Viglianti

23 Innate Immunity, Toll-Like Receptors and Atherosclerosis: Mouse Models and Methods
Rosalinda Sorrentino and Moshe Arditi

24 Generation of Parasite Antigens for Use in
In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories. In Toll-Like Receptors: Methods and Protocols , experts in the field contribute techniques currently used to study TLRs, their downstream signalling pathways, and their role in the pathogenesis of disease, with sections examining TLR expression and the application of genetic techniques and microarray analysis to TLR research. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections highlighting tips on troubleshooting and avoiding known pitfalls.
Versatile and cutting-edge, Toll-Like Receptors: Methods and Protocols provides an ideal manual for a wide range of biologists and medical researchers studying these vital components of the immune system.