The Nuclear Receptor Superfamily
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The Nuclear Receptor Superfamily

Methods and Protocols
 Previously published in hardcover
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Previously published in hardcover
Iain J. McEwan
717 g
280x210x15 mm

In this book, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors. Clear step-by-step comprehensive guides cover cutting-edge methodologies.
Provides an easily accessible reference for a range of biochemical and cell-based methods to study nuclear receptor function
Part I: Introduction
1. Nuclear Receptors: One Big Family
Iain J. McEwan
Part II: Ligand Binding and Nuclear Receptor Turnover
2. Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells
Elizabeth M. Wilson
3. Flow Cytometry as a Tool for Measurement of Steroid Hormone Receptor Protein Expression in Leukocytes
Cherie L. Butts and Esther M. Sternberg
4. X-ray Crystallography of Agonist / Antagonist-Bound Receptors
Ashley C.W. Pike
Part III: Nuclear Localization and DNA Binding
5. FRAP and FRET Methods to Study Nuclear Receptors in Living Cells
Martin E. van Royen, Christoffel Dinant, Pascal Farla, J. Trapman, and Adriaan B. Houtsmuller
6. Receptor-DNA Interactions: EMSA and Footprinting
Jason T. Read, Helen Cheng, Stephen C. Hendy, Colleen C. Nelson, and Paul S. Rennie
7. Chromatin Immunoprecipitation (ChIP) Methodology and Readouts
Charles E. Massie and Ian G. Mills
Part IV: Nuclear Receptor-Co-regulatory Protein Interactions
8. Yeast-Based Reporter Assays for the Functional Characterization of Cochaperone Interactions with Steroid Hormone Receptors
Heather A. Balsiger and Marc B. Cox
9. High Throughput Analysis of Nuclear Receptor-Cofactor Interactions
Michael L. Goodson, Behnom Farboud and Martin L. Privalsky
10. Binding Affinity and Kinetic Analysis of Nuclear Receptor/Co-Regulator Interactions Using Surface Plasmon Resonance
Derek N. Lavery
11. Using RNA Interference to Study Protein Function
Carol D. Curtis and Ann M. Nardulli
12. Using Intrinsic Fluorescence Emission Spectroscopy to Study Steroid Receptor and Co-Activator Protein Conformation Dynamics
Kate Watt and Iain J. McEwan
Part V: Pathophysiological Analysis of Nuclear Receptor Function
13. Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors
Inés Pineda Torra, Julia A. Staverosky, Susan Ha, Susan K. Logan, and Michael J. Garabedian
14. Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action
Karel De Gendt and Guido Verhoeven
15. Methods for Identifying and Studying Genetic Alterations in Hormone-Dependent Cancers
Outi R. Saramäki, Kati K. Waltering,and Tapio Visakorpi
Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this ever-growing field of study.