Germline Stem Cells
-12 %

Germline Stem Cells

 Previously published in hardcover
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ISBN-13:
9781617378805
Einband:
Previously published in hardcover
Seiten:
288
Autor:
Steven X. Hou
Gewicht:
439 g
Format:
235x155x15 mm
Sprache:
Englisch
Beschreibung:

The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells. Following the highly successful Methods in Molecular Biology series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research.
Contents Preface............................................................................................ Contributors...................................................................................... Part I Identification and Regulation of Germline Stem Cells in Model Organisms The Development of Germline Stem Cells in Drosophila David A. Dansereau and Paul Lasko................................................... Analysis of the C. elegans Germline Stem Cell Region Sarah L. Crittenden and Judith Kimble .............................................. Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell Shree Ram Singh and Steven X. Hou................................................. Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells Wei Liu and Steven X. Hou............................................................ Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (the milkweed bug Oncopeltus fasciatus) David C. Dorn and August Dorn...................................................... High Resolution Light Microscopic Characterization of Spermatogonia Hélio Chiarini-Garcia and Marvin L Meistrich...................................... Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes Jens Ehmcke and Stefan Schlatt........................................................................... Epigenetic Control in Male Germ Cells Durga Prasad Mishra and PaoloSassone-Corsi..................................... GDNF Maintains Mouse Spermatogonial Stem Cells in vivo and in vitro Hannu Sariola and Tiina Immonen..................................................... Part II In vitro Culture and Applications of Germline Stem Cells Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts Ina Dobrinski and Rahul Rathi............................................. Spermatogonial Stem Cell Transplantation, Testicular Function and Restoration of Male Fertility in Mice Derek J. McLean.............................................................................. 12 Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture F. Kent Hamra, Karen M. Chapman, Zhuoru Wu, and David L. Garbers...chrically Complex Trait Jason D. Heaney and Joseph H. Nadeau................................................ Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries Antonin Bukovsky, Satish K. Gupta, Irma Virant-Klun, Nirmala B. Upadhyaya, Pleas Copas, Stuart E. Van Meter, Marta Svetlikova, Maria E. Ayala, and Roberto Dominguez .............................................................. Index.....................................................................................................
The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells. Following the highly successful Methods in Molecular Biology series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research. In this comprehensive and cutting-edge book, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. The volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.