Immunological Tolerance
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Immunological Tolerance

Methods and Protocols
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Paul J. Fairchild
728 g
229x152x mm
Vol.380, Methods in Molecular Biology

Provides up-to-date reviews of the literature on essential topics in immunological tolerance Documents methods for the generation and culture of critical cell types and approaches to their characterization Describes protocols for the study of tolerance both in vitro and in vivo Provides insights into novel techniques for tolerance induction
Immunological Tolerance: Methods and ProtocolsTable of Contents
1. Frontiers of Immunological Tolerance
Giorgio Raimondi, Heth Turnquist & Angus Thomson
University of Pittsburgh Medical Center, Thomas E Starzl Transplantation Institute, Pittsburgh, USA
Cell Types Contributing to Immunological Tolerance
2. Case Study: Balancing Tolerance & Immunity: The Role of Dendritic Cell & T Cell Subsets
Elena Chklovskaia & Barbara Fazekas de St Groth
Centenary Institute of Cancer Medicine & Cell Biology, University of Sydney, Sydney, Australia
3. Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow
Ludovica Bruno
The Institute of Cancer Research, London, UK
4. Genetic Modification of Dendritic Cells through the Directed Differentiation of Embryonic Stem Cells
Paul J. Fairchild, Kathleen F. Nolan, &Herman Waldmann
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
5. Generation of Immunocompetent T Cells from Embryonic Stem Cells
Renée F. de Pooter & Juan-Carlos Zuñiga-Pflücker Dept of Immunology, University of Toronto, Sunnybrook & Women's Research Institute, Toronto, Canada
6. Isolation, Expansion and Characterization of Human Natural and Adaptive Regulatory T Cells
Silvia Gregori, Rosa Bacchetta,Laura Passerini, Megan K. Levings & Maria Grazia Roncarolo
San Raffaele Telethon Institute for Gene Therapy, Milan, Italy
7. Derivation, Culture and Characterization of Thymic Epithelial Cell Lines
Michiyuki Kasai & Toshiaki Mizuochi
Dept of Research on Blood & Biological Product, National Institute of Infectious Diseases, Tokyo, Japan
The Study of Immunological Tolerance in Vitro
8. Case Study: Thymus Organogenesis and Development of the Thymic Stroma
Craig Scott Nowell, Alison Mary Farley & Catherine Clare Blackburn
Institute for Stem Cell Research, University of Edinburgh, Edinburgh, UK
9. Case Study: Generation of a Tissue Engineered Thymic Organoid
Fabrizio Vianello & Mark C. Poznansky
Infectious Diseases Division & Partners AIDS Research Center, Harvard Medical School, Charlestown, USA
10. Studying T Cell Repertoire Selection Using Fetal Thymus Organ Cultures
Philip G. Ashton-Rickardt
Department of Pathology & Ben May Institute for Cancer Research, University of Chicago, Chicago, USA
11. Investigating Central Tolerance with Reaggregate Thymus Organ Cultures
Graham Anderson & Eric J. Jenkinson
MRC Centre for Immune Regulation, Department of Anatomy, University of Birmingham, Birmingham, UK
12. Estimating Thymic Function through Quantification of T Cell Receptor Excision Circles
Marie-Lise Dion, Rafick-Pierre Sékaly & Rémi Cheynier
Laboratoire d'Immunologie, Centre de Recherches du CHUM, Montréal, Canada
13. Gene Expression Profiling of Dendritic Cells by Microarray
Maria Foti, Paola Ricciardi-Castagnoli & Francesca Granucci
Dept of Biotechnology and Bioscience, University of Milano-Bicocca, Milan, Italy
14. SAGE Analysis of Cell Types Involved in Tolerance Induction
Kathleen F. Nolan, Stephen P. Cobbold & Herman Waldmann
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
15. Analyzing the Physicodynamics of Immune Cells in a 3-D Collagen Matrix
Peter Reichardt, Frank Gunzer & Matthias Gunzer
German Research Centre for Biotechnology, Braunschweig, Germany
The Study of Immunological Tolerance in Vivo
16. Case Study: Aetiology of Autoimmune Disease: How T Cells Escape Self-Tolerance
Eli Sercarz & Claudia Raja-Gabaglia
Torey Pines Institute for Molecular Studies, San Diego, USA
17. Case Study: Animal Models of Spontaneous Autoimmune Disease: Type 1 Diabetes
Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo. This guide is aimed at AIDS researchers, immunologists, transplant surgeons/specialists, and biotechnologists.