Plant Proteomics
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Plant Proteomics

Methods and Protocols
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Hervé Thiellement
596 g
229x152x mm
Vol.355, Methods in Molecular Biology

Total Protein Extraction with TCA-AcetoneValérie Méchin, Catherine Damerval and Michel Zivy Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues
Mireille Faurobert, Esther Pelpoir and Jamila Chaïb
Protein Extraction from Cereal Seeds
Gérard Branlard and Emmanuelle Bancel
Protein Extraction from Xylem and Phloem Sap
Julia Kehr and Martijn Rep
Protein Extraction from Woody Plants
Christophe Plomion and Céline Lalanne
Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis
Klaas J. van Wijk, Jean-Benoit Peltier and Lisa Giacomelli
Isolation and Sub-Fractionation of Plant Mitochondria for Proteomic Analysis
Holger Eubel, Joshua L. Heazlewood and A. Harvey Millar
Extraction of Nuclear Proteins from Root Meristematic Cells
Fernando González-Camacho and Francisco Javier Medina
Isolation of Nuclear Proteins
Setsuko Komatsu
Isolation of Cell Wall Proteins from Medicago sativa Stems
Bonnie S. Watson and Lloyd W. Sumner
Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis
Véronique Santoni
Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis
Thierry Rabilloud, Sylvie Luche, Véronique Santoni and Mireille Chevallet
Two-Dimensional Electrophoresis for Plant Proteomics
Walter Weiss and Angelika Görg
Visible and Fluorescent Staining of Two-Dimensional Gels
François Chevalier, Valérie Rofidal and Michel Rossignol
Two-Dimensional Differential In-Gel Electrophoresis (DIGE) of Leaf and Roots of Lycopersicon esculentum
Matthew Keeler, Jessica Letarte, Emily Hattrup, Fatimah Hickman and Paul A. Haynes
Quantitative Analysis of 2D Gels
Michel Zivy
Multivariate Data Analysis of Proteome Data
KåreEngkilde, Susanne Jacobsen and Ib Søndergaard
Edman Sequencing of Plant Proteins from 2D Gels
Setsuko Komatsu
Peptide Mass Fingerprinting, Identification of Proteins by Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF)
Nicolas Sommerer, Delphine Centeno and Michel Rossignol
Protein Identification using Nano Liquid Chromatography: Tandem Mass Spectrometry
Luc Negroni
Two-Dimensional Nanoflow Liquid Chromatography: Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots
Linda Breci and Paul A. Haynes
Separation, Identification and Profiling of Membrane Proteins by GFC/ IEC/SDS-PAGE and MALDI TOF MS
Wojciech Szponarski, Frédéric Delom, Nicolas Sommerer, Michel Rossignol and Rémy Gibrat
The PROTICdb Database for 2-DE Proteomics
Olivier Langella, Michel Zivy and Johann Joets
Identification of Phosphorylated Proteins
Maria V. Turkina and Alexander V. Vener
Plant Proteomics and Glycosylation
Anne-Catherine Fitchette, Olivia Tran Dinh, Loïc Faye and Muriel Bardor
Blue-Native Gel Electrophoresis for the Characterization of Protein Complexes in Plants
Jesco Heinemeyer, Dagmar Lewejohann and Hans-Peter Braun
Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis
Zhentian Lei, Ajith Anand, Kirankumar S. Mysore and Lloyd W. Sumner
Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions
Birgit Kersten and Tanja Feilner
Phosphorylation Studies Using Plant Protein Microarrays
Tanja Feilner and Birgit Kersten
The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).