Epidermal Growth Factor
-12 %

Epidermal Growth Factor

Methods and Protocols
 Previously published in hardcover
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ISBN-13:
9781617375576
Einband:
Previously published in hardcover
Erscheinungsdatum:
09.12.2010
Seiten:
220
Autor:
Paul J. Bertics
Gewicht:
324 g
Format:
229x152x12 mm
Sprache:
Englisch
Beschreibung:

Epidermal growth factor (EGF) and related molecules regulate a wide array of biological events, ranging from normal growth and development to such pathological states as cancer and cardiovascular diseases. In Epidermal Growth Factor: Methods and Protocols, expert researchers active in the field present their best methods to dissect the mechanisms that control growth factors and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and highly practical, Epidermal Growth Factor: Methods and Protocols offers investigators an array of powerful and proven tools to unravel the workings of epidermal growth factor, as well as develop new strategies for the successful use of this important developmental and therapeutic target.
A Historical Perspective of the EGF Receptor and Related SystemsFrancis Edwin, Gregory J. Wiepz, Rakesh Singh, Chimera Rene Peet, Deepti Chaturvedi, Paul J. Bertics, and Tarun B. Patel
Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromatography
Gregory J. Wiepz, Arturo G. Guadaramma, David L. Fulgham, and Paul J. Bertics
Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay
Jacqueline M. Lafky, Andre T. Baron, and Nita J. Maihle
Analysis of EGF Receptor Interactions With the a Subunit of the Stimulatory GTP Binding Protein of Adenylyl Cyclase, Gs
Deepti Chaturvedi, Francis Edwin, Hui Sun, and Tarun B. Patel
Regulator of Epidermal Growth Factor Signaling: Sprouty
Esther Sook Miin Wong and Graeme R. Guy
Dissecting the Epidermal Growth Factor Receptor Signal Transactivation Pathway
Oliver M. Fischer, Stefan Hart, and Axel Ullrich
A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor
Umut Sahin, Gisela Weskamp, Yufang Zheng, Valerie Chesneau, Keisuke Horiuchi, and Carl P. Blobel
In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases
Menachem Katz, Yaron Mosesson, and Yosef Yarden
Assays to Monitor Degradation of the EGF Receptor
Mirko H. H. Schmidt and Ivan Dikic
Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members
Adriano Marchese
Epithelial Cell Migration in Response to Epidermal Growth Factor
Reema Zeineldin and Laurie G. Hudson
Motility Signaled From the EGF Receptor and Related Systems
Alan Wells, Brian Harms, Akihiro Iwabu, Lily Koo, Kirsty Smith, Linda Griffith, and Douglas A. Lauffenburger
Methods for Determining the Proliferation of Cells in Response to EGFR Ligands
Gregory J. Wiepz, Francis Edwin, Tarun B. Patel, and Paul J. Bertics
Clinical Advancement of EGFR Inhibitors in Cancer Therapy
Prakash Chinnaiyan and Paul M. Harari
Index
A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.