Protein Engineering
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Protein Engineering

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ISBN-13:
9780471103547
Einband:
Buch
Erscheinungsdatum:
20.03.1996
Seiten:
544
Autor:
Jeffrey L. Cleland
Gewicht:
1170 g
Format:
260x183x32 mm
Sprache:
Englisch
Beschreibung:
This provides a thorough introduction to protein biophysics via chapters dealing with experimental and theoretical determination of protein structure. It discuss each of the various expressions systems currently available and explore the relative advantages and limitations of each system, it also cover the difficulty in achieving correctly folded and functional proteins, the relationship between the structure and function of proteins, and specific examples of the use of protein engineering in construction of synthetic metal binding sites in proteins, the manufacture of tissue plasminogen activatior, and the generation of therapeutic antibodies.
This provides a thorough introduction to protein biophysics via chapters dealing with experimental and theoretical determination of protein structure. It discuss each of the various expressions systems currently available and explore the relative advantages and limitations of each system, it also cover the difficulty in achieving correctly folded and functional proteins, the relationship between the structure and function of proteins, and specific examples of the use of protein engineering in construction of synthetic metal binding sites in proteins, the manufacture of tissue plasminogen activatior, and the generation of therapeutic antibodies.
Partial table of contents:
Protein Conformation (F. Cohen & D. Hearst).
Predicting the Conformation of Proteins from Sequence Data (S. Benner).
Expression of Proteins in Bacteria (G. Georgiou).
Expression of Heterologous Gene Products in Yeast (S. Pichuantes, et al.).
Insect Cell Expression Technology (V. Luckow).
Protein Expression in Xenopus Oocytes (M. Marino).
Protein Engineering for Stability (S. Braxton).
Structure-Function Relationships for Protein Design (C. Gibbs).
Bacteriophage Display Libraries (J. Winter).
Engineering Therapeutic Antibodies (R. Kelley).
Site-Directed Drug Design (D. DeCamp, et al.).
Index.
PROTEIN ENGINEERING Principles and Practice
Edited by JEFFREY L. CLELAND CHARLES S. CRAIK

Proteins are involved in every aspect of life-structure, motion, catalysis, recognition and regulation. Protein Engineering: Principles and Practice provides a basic framework for understanding both proteins and protein engineering. This comprehensive book covers general, yet essential knowledge required for successful protein engineering, including everything from the fundamentals to modifying existing proteins and developing new proteins.

The book begins by introducing the main concepts of protein engineering, including: understanding protein conformation, comprehending the relationship between protein composition and structure, and potential methods for predicting a protein s conformation.

Other major subjects addressed are:
Using different host cell expression systems to produce specific proteins
Protein folding
Structure and function of proteins in relation to drug design
Construction of synthetic metal binding sites in proteins
Manufacture of tissue plasminogen activator
Generation of therapeutic antibodies

This broad range of topics provides a solid foundation in protein engineering and supplies readers with knowledge essential to the design and production of proteins.

Of primary interest to protein scientists-both students and researchers, in academia as well as industry-Protein Engineering is also extremely useful to chemical engineers, protein chemists, biochemists, and pharmaceutical chemists.